ACES, Buffer

ACES buffer (N-(2-Acetamido)-2-aminoethanesulfonic acid) is a zwitterionic biological buffer that is widely used in biochemistry and molecular biology experiments. It has a pika value of 7.0, making it an effective buffer at pH levels ranging from 6.1 to 7.5. ACES buffers were first introduced in 1975 as a replacement for the widely used Good’s buffers. ACES, unlike Good’s buffers, lacks carboxylic acid groups, which are susceptible to oxidation and other chemical modifications. As a result, ACES is more stable and less likely to disrupt biological reactions. ACES buffer is frequently used in electrophoresis and other biochemical experiments requiring a constant ph. It is also commonly used in protein purification and crystallization, where a stable pH is required to keep the protein active and stable. The ability of ACES buffer to stabilize enzymes and other biomolecules that are sensitive to pH changes is one of its advantages. ACES buffer has been shown to be effective at enzyme stabilization, including proteases, lipases, and peroxidases. This makes it an excellent buffer for a wide range of biochemical and biotechnological applications.